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Phytoplankton Analysis

Solicitation Number: R10PS80803
Agency: Department of the Interior
Office: Bureau of Reclamation
Location: BOR - All Offices
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Combined Synopsis/Solicitation
Added: Aug 14, 2010 9:02 am
Phytoplankton analysis on field collected phytoplankton samples from Lake Mead, AZ-NV.
This is a combined synopsis/solicitation for commercial service prepared in accordance with the format in Subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; proposals are being requested and a written solicitation will not be issued.
(ii) This solicitation, R10PS80803 is issued as a request for proposal (RFP).
(iii) This solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisition Circular 2005-43.
(iv) This solicitation is 100% small business set-aside. NAICS Code is 541620. Size standard is $7.0 millions of dollars.
(v) This combined synopsis/solicitation is for the following commercial services: Phytoplankton analysis on field collected phytoplankton samples from Lake Mead, AZ-NV.
(vi) Description of requirements for the services to be acquired: For 240 samples of Phytoplankton analyses are required on field-collected phytoplankton samples from Lake Mead, AZ-NV. Samples will be collected on a monthly basis, preserved with glutaraldyde, and shipped to the contractor. In order to maintain consistency with the established database, it is essential that the contractor use procedures that are the same as those that have been used for the past twenty (20) years.
Phytoplankton need to be identified to genus, and species where possible. Results must to be reported on a monthly basis for all stations sampled, both as cell counts (cells/mL) and biovolume (m3/mL). Raw data must be provided in Excel spreadsheets, with algal genera sorted by division for each sampling station. For presentation purposes, both algal cell counts and biovolumes must also be lumped into the following major groups: Diatoms (Bacillariophyta), Green algae (Chlorophyta), Cryptomonads (Cryptophyta), Yellow-brown algae (Chrysophyta), Blue-green algae (Cyanophyta), Euglenoids (Euglenophyta), Dinoflagellates (Pyrrhophyta), Miscellaneous algae (includes microflagellates, unidentifiable cysts, and Gonyostomum semen, which is in the division Chloromonadophyta).
Samples are to be processed using a research-grade compound microscope equipped with variable optics, including Phase Optics (400x), epifluorescence, and a trinocular head for photography. The magnification used will depend on the size of dominant taxa, and the size and number of particulates. Counting is to be done at multiple magnifications to correctly enumerate and identify taxa present that can vary by several orders of magnitude in size. If samples are dominated by cells below 10-20 ?m, or if cells are fragile and difficult to identify, the majority of counting will be completed at 400x-1000x. A minimum of 400 natural units per sample will be enumerated, exclusive of miscellaneous microflagellates. QA/QC will be performed on approximately every tenth sample for random distribution and identification verification.
Biovolume measurements will be completed by measuring the greatest axial linear dimension (GALD), which is the length, width and depth of different aspects of the colony or cell. Cell and colony shapes will be approximated to a geometric figure and or figures and the appropriate calculations made. These measurements provide a general indication of the edibility of an algal cell for zooplankton, with GALD measurement of <30 m usually considered edible and GALD >30 m usually considered inedible. Up to a total of 30 natural units (sometimes higher on exceptionally variable taxa) will be measured for each taxa depending on variability and number encountered. The use of literature values for biovolume calculations is not acceptable.
Raw data are to be recorded on count sheets and transferred to a data analysis and management program. The program should retain original input data for future reference and error checking.
Permanent mounts should be prepared for each sample and maintained in an archive in case questions arise concerning species identification. While this increases analytical costs, results from the Lake Mead limnology project have the potential to affect billions of dollars in future construction and access to archived samples could prove to be extremely useful if questions on changes in algal characteristics arise.
The HPMA method (Crumpton, 1987, a simple and reliable method for making permanent mounts of phytoplankton for light and fluorescence microscopy. Limnol. Oceanogr. 32: 1154-1159) should be used to produce the archival algal sample slides because it provides an optically clear background while permanently infiltrating and preserving the sample for archival purposes. Original samples should be retained in case clarification or further identification is necessary.
The initial award will be for samples collected during calendar year 2010. Up to two additional option years may be included, depending upon Reclamation's ability to obtain funding for future sampling. Approximately twenty (20) samples will be collected each month, with a maximum of 250 samples collected during the year.
(vii) This work should start in September-October 2010. The work to be performed is located in section (v).
(viii) The provision at FAR 52.212-1, Instructions to Offerors-Commercial, applies to this acquisition.
(ix) The provision at FAR 52.212-2, Evaluation -- Commercial Items, applies to this acquisition. The Government will award a fixed price purchase order to the best value offeror conforming to the terms and requirements of the solicitation.
1) Technical Capability - The offeror's proposal will be evaluated to the extent the technical capability demonstrates experience and proficiency in 1) Previous experience with algal identification and enumeration, 2) Previous experience on providing data for long-term monitoring programs, 3) Previous experience with the preparation of archival slides, 4) Previous experience with algal identification at Lake Mead, which is necessary to maintain continuity with existing monitoring programs.
2) Past Performance - Past performance evaluation will focus on the quality of previous work produced by the offeror. Key considerations include: (1) collection of phytoplankton samples 2) satisfactory performance in collection of phytoplankton samples under Federal contract. This factor is less important when compared to technical capability.
3) Price- Technical capability and past performances, when combined, are significantly more important when compared to price.
The relative order of all evaluation factors is (1) Technical Capability, (2) Past Performance, (3) Price. When combined technical capability, past performance are significantly more important than price.
(x) The offeror must have completed the Online Representations and Certifications Application (ORCA) which is the provision at FAR 52.212-3, Offeror Representations and Certifications -- Commercial Items. The web site address is
(xi) The provision at FAR 52.212-4, Contract Terms and Conditions -- Commercial Items, applies to this acquisition.
(xii) The provision at FAR 52.212-5, Contract Terms and Conditions Required Implementing Statutes or Executive Orders -- Commercial Items applies to this acquisition. Additional FAR 52.212-5 clauses cited in the clause applicable to this acquisition are, b (19), b (21), b (22), b (23), b (24), b (25), b (26), b (38).
(xiii) The provision at Department of Interior 1452.215-71- Use and Disclosure of Proposal Information applies to this acquisition.
(xiv) Defense Priorities and Allocations System (DPAS) ratings do not apply to this acquisition.
(xv) Proposals are required to be received no later than 4:00 pm Mountain Standard Time, Friday September 03, 2010, and must include the following: Company name, address phone numbers, DUNS number, Tax ID number, proposals shall be delivered via email to
(xvi) Please direct any questions regarding this solicitation to Al Phillips at 303-445-2449 or email
Bureau of Reclamation-DO-Acquisition Operations Group PO Box 25007, 84-27810 Denver CO 80225
Lake Mead
Al Phillips Contract Specialist 3034452449;

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